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KMID : 1094020060230040411
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Journal of Veterinary Clinics
2006 Volume.23 No. 4 p.411 ~ p.415
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Effects of Ascorbic Acid on the Proliferation of Subcultured Canine Chondocytes in Monolayer and Alginate Beads Culture
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Kim Gon-Hyung
Park Jin-Uk
Hossain Mohammad Alamgir
Cho Ki-Rae
Kim Joong-Hyun
Choi Seok-Hwa
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Abstract
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Ascorbic acid has been used widely as a medium supplement to stimulate cell proliferation, but its effects on cell proliferation have not yet been elucidated, and no reports have analyzed effects on subcultured chondrocytes. Subcultured canine chondrocytes of passage one, two and four were cultured in monolayer and alginate beads with and without ascorbic acid. Cell proliferation was examined by 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt) (XTT) colormetric assay. Ascorbic acid stimulated cell proliferation significantly in both culture methods (p<0.05). The increased cell numbers by stimulation with ascorbic acid were significantly high in passage one cells compared to that of other passages. Differences in cell proliferative capacity by subculturing were not determined. These results suggest that ascorbic acid stimulated the proliferation of subcultured canine chondrocytes and enhanced it more in low-passage cells than in the other cells tested.
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KEYWORD
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Ascorbic acid, Cell proliferation, Chondrocytes, Dog
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